Journal: medRxiv
Article Title: Functional profiling of human chorionic gonadotrophin in embryo peri- and post-implantation in vitro models
doi: 10.64898/2026.04.01.26349947
Figure Lengend Snippet: LHCGR activation assay in the peri-implantation model. A-C , Representative examples of cAMP-sensor signal change (ΔFRET) after addition of the hCG-containing samples at time t = 0 s. The type of treatment is indicated above the graph; for the conditioned medium samples ( A – B ), the dilutions used are indicated on the right side, and for the Ovitrelle control ( C ), the concentration of the recombinant hCG is listed under the graph. The kinetic curves show data from a single experiment; error bars indicate standard deviation of technical triplicates. D , Biological activity of hCG in conditioned culture media from the peri-implantation model. Based on the LHCGR-mediated increase in intracellular cAMP levels, hCG concentration in each sample was recalculated from comparison with recombinant hCG standard (Ovitrelle). Sample treatment types are listed under the graph (OFEL stands for open-faced endometrial layer, EP for hormonal stimulation and 5B stands for 5 blastoids); average hCG level ± standard error of mean is shown (for 2 independent experiments, each experiment using OFEL from 2 different donors). Statistical significance of grouped comparisons (ANOVA with Dunnett’s correction for multiple comparisons): ** P < 0.01, * P < 0.05, ns – not significant.
Article Snippet: Recombinant hCG (Ovitrelle) was used as a positive control (Merck Serono Europe, Germany).
Techniques: Activation Assay, Control, Concentration Assay, Recombinant, Standard Deviation, Activity Assay, Comparison
